Soo KoobidTijaabada waxaa la sameeyay si loo barto saameynta diludine ee waxqabadka dhigista iyo tayada ukunta ee digaagga iyo habka loo wajaho habka saamaynta iyadoo la go'aaminayo tusmooyinka xuduudaha ukunta iyo seeramka. 1024 Digaagga ROM waxaa loo qaybiyay afar kooxood midkiiba oo ay ku jiraan afar nuqul oo ah 64 digaag midkiiba. Kooxaha daaweynta waxay heleen cunto isku mid ah oo lagu daray 0, 100, 150, 200 mg/kg diludine siday u kala horreeyaan muddo 80 maalmood ah. Natiijooyinku waxay ahaayeen sidan soo socota. Ku darista diludine cuntada waxay wanaajisay waxqabadka dhigista digaagga, kuwaas oo daawaynta 150 mg/kg ay ugu fiicnayd; heerka jiifku wuxuu kordhay 11.8% (p< 0.01), beddelka tirada ukunta ayaa hoos u dhacay 10.36% (p< 0 01). Miisaanka ukunta ayaa la kordhiyay iyadoo la kordhinayo diludine lagu daray. Diludine si weyn ayuu u yareeyay fiirsashada serumka ee uric acid (p< 0.01); ku darista diludine si weyn ayuu hoos ugu dhigay serumka Ca²⁺iyo heerka fosfate-ka aan dabiiciga ahayn, iyo kororka dhaqdhaqaaqa alkine phosphatase (ALP) ee seeramka (p< 0.05), sidaa darteed waxay saameyn weyn ku yeelatay yareynta jabka ukunta (p<0.05) iyo cilladda (p < 0.05); diludine si weyn ayuu u kordhiyay dhererka albumen. Qiimaha Haugh (p <0.01), dhumucda qolofka iyo miisaanka qolofka (p< 0.05), 150 iyo 200mg/kg diludine ayaa sidoo kale hoos u dhigay wadarta guud ee kolesteroolka ee jaallaha ukunta (p< 0 05), laakiin waxay kordhisay miisaanka jaallaha ukunta (p < 0.05). Intaa waxaa dheer, diludine wuxuu kor u qaadi karaa dhaqdhaqaaqa lipase (p < 0.01), wuxuuna yareyn karaa heerka triglyceride (TG3) (p< 0.01) iyo kolestaroolka (CHL) (p< 0 01) ee seeramka, waxay hoos u dhigtay boqolkiiba dufanka caloosha (p< 0.01) iyo heerka dufanka beerka (p< 0.01), waxay awood u lahayd inay ka hortagto digaagga beerka dufanka leh. Diludine wuxuu si weyn u kordhiyay firfircoonida SOD ee seeramka (p< 0 01) markii lagu daray cuntada in ka badan 30 maalmood. Si kastaba ha ahaatee, ma jirin wax farqi ah oo muhiim ah oo laga helay hawlaha GPT iyo GOT ee seeramka u dhexeeya kooxda la xakameeyey iyo kooxda la daweeyay. Waxaa la soo jeediyay in diludine uu ka hortagi karo xuubka unugyada oksaydheynta.

Erayada Muhiimka ahDiludine; digaag; SOD; kolestarool; triglyceride, lipase

 

Diludine waa fiitamiin cusub oo ka hortaga oksaydhka oo aan nafaqo lahayn wuxuuna leeyahay saameyn^[1-3]xakamaynta oksaydhka xuubka bayoolojiga iyo xasilinta unugyada bayoolojiga, iwm. Sannadkii 1970-meeyadii, khabiirka beeraha ee Latvia ee Midowgii Soofiyeeti ee hore wuxuu ogaaday in diludine uu leeyahay saameynta^[4]kor u qaadista koritaanka digaagga iyo iska caabbinta barafowga iyo gabowga dhirta qaar. Waxaa la soo sheegay in diludine-ku uusan kaliya kor u qaadi karin koritaanka xayawaanka, laakiin uu si cad u wanaajin karo waxqabadka taranka ee xayawaanka iyo hagaajinta heerka uurka, soo saarista caanaha, soo saarista ukunta iyo heerka dillaaca ee xayawaanka dheddigga ah.^{[1, 2, 5-7]}Daraasadda diludine ee Shiinaha waxaa la bilaabay 1980-meeyadii, inta badan daraasadaha ku saabsan diludine ee Shiinaha waxay ku kooban yihiin isticmaalka saameynta ilaa hadda, waxaana la soo sheegay tijaabooyin yar oo ku saabsan digaagga dhalaya. Chen Jufang (1993) wuxuu sheegay in diludine-ku uu hagaajin karo wax soo saarka ukunta iyo miisaanka ukunta digaagga, laakiin ma uusan sii dheeraan.^[5]Daraasadda habka ficilkeeda. Sidaa darteed, waxaan hirgelinay daraasad nidaamsan oo ku saabsan saameynta iyo habka ay u shaqeyso annagoo digaagga jiifa ku quudinay cunto lagu daray diludine, qayb ka mid ah natiijadana hadda waxaa lagu soo waramayaa sidan soo socota:

Shaxda 1aad Halabuurka iyo qaybaha nafaqada ee cuntada tijaabada ah

%

---------------------------------------------------------------------------------------------------------------------------

Halabuurka cuntada Qaybaha nafaqada

---------------------------------------------------------------------------------------------------------------------------

Galley 62 ME③ 11.97

Saxanka Digirta 20 CP 17.8

Cunto kalluun 3 Ca 3.42

Cuntada Rapeseed 5 P 0.75

Cunto lafaha 2 M et 0.43

Cunto dhagax ah 7.5 M iyo Cys 0.75

Methionine 0.1

Cusbo 0.3

Faytamiino badan① 10

Walxaha raad-raaca② 0.1

---------------------------------------------------------------------------------------------------------------------------------

① Fiitamiinno badan: 11mg oo riboflavin ah, 26mg oo folic acid ah, 44mg oo oryzanin ah, 66mg oo niacin ah, 0.22mg oo biotin ah, 66mg oo B6 ah, 17.6ug oo B12 ah, 880mg oo choline ah, 30mg oo VK ah, 66IU oo V ah_E, 6600ICU ee V_Diyo 20000ICU ee V_A, ayaa lagu daraa kiilo kasta oo cuntada ah; 10g oo fiitamiinno badan ah ayaa lagu daraa 50kg kasta oo cunto ah.

② Curiyeyaasha raadadka (mg/kg): 60 mg oo Mn ah, 60mg oo Zn ah, 80mg oo Fe ah, 10mg oo Cu ah, 0.35mg oo I ah iyo 0.3mg oo Se ah ayaa lagu daraa kiilo kasta oo cuntada ah.

③ Cutubka tamarta la dheefshiidi karo waxa loola jeedaa MJ/kg.

 

1. Qalabka iyo habka

1.1 Qalabka tijaabada

Beijing Sunpu Biochem. & Tech. Co., Ltd. waa inay bixisaa diludine; xayawaanka tijaabada ahna wuxuu tixraaci doonaa digaagga ganacsiga ee Roomaanka ee 300 maalmood jira.

 

Cunto tijaabo ah: cuntada tijaabada ah waa in loo diyaariyaa iyadoo loo eegayo xaaladda dhabta ah inta lagu jiro wax soo saarka iyadoo lagu saleynayo heerka NRC, sida ku cad Shaxda 1.

1.2 Habka tijaabada

1.2.1 Tijaabada quudinta: Tijaabada quudinta waa in lagu hirgeliyaa beerta Shirkadda Hongji ee Magaalada Jiande; 1024 Digaag Roomaan ah oo la dhigo waa in la doortaa oo loo qaybiyaa afar kooxood si aan kala sooc lahayn midkiibana 256 xabbo (koox kasta waa in lagu celiyaa afar jeer, digaag kastana waa in lagu celiyaa 64 jeer); digaagga waa in lagu quudiyaa afarta cunto ee leh waxyaabo kala duwan oo diludine ah, iyo 0, 100, 150, 200mg/kg oo quudin ah waa in lagu daraa koox kasta. Tijaabada waxaa la bilaabay Abriil 10, 1997; digaaggana waxay heli kareen cunto oo ay si xor ah u qaadan kareen biyaha. Cuntada ay qaataan koox kasta, heerka dhalmada, wax soo saarka ukunta, ukunta jabtay iyo tirada ukunta aan caadiga ahayn waa in la diiwaangeliyaa. Intaa waxaa dheer, tijaabada waxaa la dhammeeyay Juun 30, 1997.

1.2.2 Cabbirka tayada ukunta: 20 ukun waa in si aan kala sooc lahayn loo qaataa marka baaritaanka la fuliyo afar maalmood oo 40 ah si loo cabbiro tilmaamayaasha la xiriira tayada ukunta, sida tusmada qaabka ukunta, cutubka haugh, miisaanka qaraabada ah ee qolofta, dhumucda qolofta, tusmada jaallaha, miisaanka qaraabada ah ee jaallaha, iwm. Intaa waxaa dheer, waxa ku jira kolestaroolka jaallaha waa in lagu cabbiraa iyadoo la adeegsanayo habka COD-PAP iyadoo la joogo reagent Cicheng oo ay soo saarto Ningbo Cixi Biochemical Test Plant.

1.2.3 Cabbirka tusmada biochemical-ka ee seeramka: 16 digaag oo tijaabo ah waa in laga qaataa koox kasta mar kasta oo baaritaanka la fulinayo muddo 30 maalmood ah iyo marka baaritaanka la dhammeeyo si loo diyaariyo seeramka ka dib marka laga qaado dhiigga xididka baalasha. Seeramka waa in lagu kaydiyaa heerkulka hooseeya (-20℃) si loo cabbiro tusmooyinka biochemical ee khuseeya. Boqolleyda dufanka caloosha iyo dufanka beerka waa in la cabbiraa ka dib marka la qalo oo laga saaro dufanka caloosha iyo beerka marka la dhammeeyo muunadda dhiigga.

Waa in lagu cabbiraa superoxide dismutase (SOD) iyadoo la adeegsanayo habka dherjinta iyadoo la joogo qalabka falgalka ee ay soo saarto Machadka Cilmi-baarista Beijing Huaqing Biochem. & Tech. Aashitada uric acid (UN) ee ku jirta seeramka waa in lagu cabbiraa iyadoo la adeegsanayo habka U ricase-PAP iyadoo la joogo qalabka falgalka Cicheng; triglyceride (TG3) waa in lagu cabbiraa iyadoo la adeegsanayo habka hal-tallaabo ee GPO-PAP iyadoo la joogo qalabka falgalka Cicheng; lipase waa in lagu cabbiraa iyadoo la isticmaalayo nephelometry iyadoo la joogo qalabka falgalka Cicheng; kolestaroolka guud ee serumka (CHL) waa in lagu cabbiraa iyadoo la adeegsanayo habka COD-PAP iyadoo la joogo qalabka falgalka Cicheng; glutamic-pyruvic transaminase (GPT) waa in lagu cabbiraa iyadoo la isticmaalayo midabka marka la joogo qalabka falgalka Cicheng; glutamic-oxalacetic transaminase (GOT) waa in lagu cabbiraa iyadoo la isticmaalayo midabka marka la joogo qalabka falgalka Cicheng; fosfatase alkaline (ALP) waa in lagu cabbiraa iyadoo la adeegsanayo habka heerka iyadoo la joogo qalabka falgalka Cicheng; ion-ka kaalshiyamka (Ca)²⁺) ee seeramka waa in lagu cabbiraa iyadoo la adeegsanayo habka methylthymol blue complexone iyadoo la joogo qalabka Cicheng reagent; fosfooraska aan organic-ga ahayn (P) waa in lagu cabbiraa iyadoo la adeegsanayo habka buluugga molybdate iyadoo la joogo qalabka Cicheng reagent.

 

2 Natiijada tijaabada

2.1 Saamaynta waxqabadka dhigista

Waxqabadka dejinta ee kooxaha kala duwan ee lagu farsameeyay isticmaalka diludine waxaa lagu muujiyay Shaxda 2.

Shaxda 2aad Waxqabadka digaagga lagu quudiyay cunto aasaasi ah oo lagu kabay afar heer oo diludine ah

 

Cadadka diludine ee lagu darayo (mg/kg)
	0	100	150	200
Qaadashada quudinta (g)
Heerka dhigidda (%)
Celceliska miisaanka ukunta (g)
Saamiga walxaha iyo ukunta
Heerka ukunta jaban (%)
Heerka ukunta aan caadiga ahayn (%)

 

Shaxda 2, heerarka dhigista ee dhammaan kooxaha lagu farsameeyo isticmaalka diludine si cad ayaa loo hagaajiyay, halkaas oo saameynta marka la farsameeyo iyadoo la isticmaalayo 150mg/kg ay tahay mid ugu wanaagsan (ilaa 83.36%), iyo 11.03% (p<0.01) la hagaajiyo marka la barbar dhigo kooxda tixraaca; sidaas darteed diludine wuxuu leeyahay saameynta hagaajinta heerka dhigista. Marka laga eego celceliska miisaanka ukunta, miisaanka ukunta ayaa sii kordhaya (p>0.05) iyadoo la kordhinayo diludine-ka cuntada maalinlaha ah. Marka la barbardhigo kooxda tixraaca, farqiga u dhexeeya dhammaan qaybaha la farsameeyay ee kooxaha lagu farsameeyo iyadoo la isticmaalayo 200mg/kg oo diludine ah ma cadda marka 1.79g oo ah qaadashada quudinta lagu daro celcelis ahaan; Si kastaba ha ahaatee, farqiga ayaa si tartiib tartiib ah u muuqda iyadoo la raacayo kororka diludine-ka, farqiga u dhexeeya saamiga walaxda iyo ukunta ee qaybaha la farsameeyayna waa mid cad (p<0.05), saameyntuna waa mid wanaagsan marka 150mg/kg oo diludine ah waana 1.25:1 kaas oo la dhimay 10.36% (p<0.01) marka la barbar dhigo kooxda tixraaca. Marka laga eego heerka ukunta ee jabtay ee dhammaan qaybaha la farsameeyay, heerka ukunta ee jabtay (p<0.05) waa la dhimi karaa marka diludine-ka lagu daro cuntada maalinlaha ah; boqolkiiba ukunta aan caadiga ahaynna waa la dhimaa (p<0.05) iyadoo la kordhinayo diludine-ka.

 

2.2 Saamaynta tayada ukunta

Marka laga eego Shaxda 3, tusmada qaabka ukunta iyo cufnaanta gaarka ah ee ukunta saameyn kuma yeelanayaan (p>0.05) marka diludine-ka lagu daro cuntada maalinlaha ah, miisaanka qolofkuna uu kordho iyadoo la kordhinayo diludine-ka lagu daray cuntada maalinlaha ah, halkaas oo miisaanka qolofka lagu kordhiyo 10.58% iyo 10.85% (p<0.05) siday u kala horreeyaan marka la barbar dhigo kooxaha tixraaca marka lagu daro 150 iyo 200mg/kg oo diludine ah; dhumucda qolofka ukunta ayaa la kordhiyaa iyadoo la kordhinayo diludine-ka cuntada maalinlaha ah, halkaas oo dhumucda qolofka ukunta la kordhiyo 13.89% (p<0.05) marka lagu daro 100mg/kg oo diludine ah marka la barbar dhigo kooxaha tixraaca, dhumucda qolofka ukuntana la kordhiyo 19.44% (p<0.01) iyo 27.7% (p<0.01) siday u kala horreeyaan marka lagu daro 150 iyo 200mg/kg. Cutubka Haugh (p<0.01) si cad ayaa loo hagaajiyaa marka diludine la daro, taasoo muujinaysa in diludine-ku uu leeyahay saameyn kor u qaadida isku-darka albumen qaro weyn oo ukunta cad. Diludine-ku wuxuu leeyahay shaqada hagaajinta tusmada jaallaha, laakiin farqigu si cad uma aha (p<0.05). Waxyaabaha ku jira kolestaroolka jaallaha ukunta ee dhammaan kooxaha waa kala duwan yihiin waxaana si cad loo dhimi karaa (p<0.05) ka dib marka lagu daro 150 iyo 200mg/kg oo diludine ah. Miisaanka jaallaha ukunta way ka duwan yihiin midba midka kale sababtoo ah xaddi kala duwan oo diludine ah oo lagu daray, halkaas oo miisaanka jaallaha ukunta la hagaajiyo 18.01% iyo 14.92% (p<0.05) marka 150mg/kg iyo 200mg/kg marka la barbar dhigo kooxda tixraaca; sidaa darteed, diludine-ka ku habboon wuxuu leeyahay saameynta kor u qaadida isku-darka jaallaha ukunta.

 

Shaxda 3aad Saamaynta diludine ee tayada ukunta

Cadadka diludine ee lagu darayo (mg/kg)
Tayada ukunta	0	100	150	200
Tusmada qaabka ukunta (%)
Cufis-jiidad gaar u ah ukunta (g/cm3)
Miisaanka qaraabada ah ee qolofta ukunta (%)
Dhumucda qolofta ukunta (mm)
Cutubka Haugh (U)
Tusmada jaallaha ukunta (%)
Kolestaroolka jaallaha ukunta (%)
Miisaanka qaraabada ah ee jaallaha ukunta (%)

 

2.3 Saamaynta boqolkiiba dufanka caloosha iyo tirada dufanka beerka ee digaagga jiifa

Fiiri Jaantuska 1aad iyo Jaantuska 2aad si aad u ogaato saameynta diludine uu ku leeyahay boqolkiiba dufanka caloosha iyo waxa ku jira dufanka beerka ee digaagga jiifa.

 

 

 

Jaantuska 1aad Saamaynta diludine ee boqolkiiba dufanka caloosha (PAF) ee digaagga dhigta

 

	Boqolleyda dufanka caloosha
	Qadarka diludine ee lagu darayo

 

 

Jaantuska 2aad Saamaynta diludine ee dufanka beerka (LF) ee digaagga dhigta

	Dufanka beerka ku jira
	Qadarka diludine ee lagu darayo

Marka laga eego Jaantuska 1, boqolleyda dufanka caloosha ee kooxda baaritaanka waxaa loo dhimay 8.3% iyo 12.11% (p<0.05) siday u kala horreeyaan marka 100 iyo 150mg/kg oo diludine ah marka la barbar dhigo kooxda tixraaca, boqolleyda dufanka calooshana waxaa loo dhimay 33.49% (p<0.01) marka lagu daro 200mg/kg oo diludine ah. Marka laga eego Jaantuska 2, waxa ku jira dufanka beerka (oo gebi ahaanba qalalan) oo lagu farsameeyay 100, 150, 200mg/kg oo diludine ah siday u kala horreeyaan ayaa loo dhimay 15.00% (p<0.05), 15.62% (p<0.05) iyo 27.7% (p<0.01) siday u kala horreeyaan marka la barbar dhigo kooxda tixraaca; Sidaa darteed, diludine-ku wuxuu leeyahay saameyn yareysa boqolkiiba dufanka caloosha iyo dufanka beerka ee ku jira maaddada la dhigay, halkaas oo saameyntu ay ugu fiican tahay marka lagu daro 200mg/kg oo diludine ah.

2.4 Saamaynta tusmada bayoolaji ee seeramka

Marka laga eego Shaxda 4, farqiga u dhexeeya qaybaha la farsameeyay inta lagu jiro Wejiga I (30d) ee baaritaanka SOD ma cadda, tusmooyinka bayoolaji ee seeramka ee dhammaan kooxaha lagu daray diludine-ka Wejiga II (80d) ee baaritaanka ayaa ka sarreeya kooxda tixraaca (p<0.05). Aashitada uric (p<0.05) ee seeramka waa la dhimi karaa marka lagu daro 150mg/kg iyo 200mg/kg oo diludine ah; halka saameynta (p<0.05) ay diyaar tahay marka lagu daro 100mg/kg oo diludine ah Wejiga I. Diludine-ku wuxuu yareyn karaa triglyceride-ka ku jira seeramka, halkaas oo saameyntu ay tahay mid ugu fiican (p<0.01) kooxda marka 150mg/kg oo diludine ah lagu daro Wejiga I, waana mid ugu fiican kooxda marka lagu daro 200mg/kg oo diludine ah Wejiga II. Kolestaroolka guud ee ku jira seeramka ayaa la dhimaa iyadoo la kordhinayo diludine-ka lagu daray cuntada maalinlaha ah, gaar ahaan waxa ku jira kolestaroolka guud ee seeramka ayaa la dhimaa 36.36% (p<0.01) iyo 40.74% (p<0.01) siday u kala horreeyaan marka 150mg/kg iyo 200mg/kg oo diludine ah lagu daro Wejiga I marka la barbar dhigo kooxda tixraaca, waxaana la dhimaa 26.60% (p<0.01), 37.40% (p<0.01) iyo 46.66% (p<0.01) siday u kala horreeyaan marka 100mg/kg, 150mg/kg iyo 200mg/kg oo diludine ah lagu daro Wejiga II marka la barbar dhigo kooxda tixraaca. Intaa waxaa dheer, ALP-ga ayaa la kordhiyaa iyadoo la kordhinayo diludine-ka lagu daray cuntada maalinlaha ah, halka qiimaha ALP ee kooxda lagu daray 150mg/kg iyo 200mg/kg ee diludine ay ka sarreeyaan kooxda tixraaca (p<0.05) si cad.

Shaxda 4 Saamaynta diludine ee xuduudaha seeramka

Cadadka diludine ee lagu darayo (mg/kg) Wajiga I (30d) ee baaritaanka
Shay	0	100	150	200
Superoxide dismutase (mg/mL)
Aashitada Uric
Triglyceride (mmol/L)
Lipase (U/L)
Kolestarool (mg/dL)
Transaminase-ka Glutamic-pyruvic (U/L)
Transaminase-ka Glutamic-oxalacetic (U/L)
Fosfatase alkaline (mmol/L)
Kaalshiyam aayoon (mmol/L)
Fosfooraska aan dabiiciga ahayn (mg/dL)

 

Cadadka diludine ee lagu darayo (mg/kg) Wajiga II (80d) ee baaritaanka
Shay	0	100	150	200
Superoxide dismutase (mg/mL)
Aashitada Uric
Triglyceride (mmol/L)
Lipase (U/L)
Kolestarool (mg/dL)
Transaminase-ka Glutamic-pyruvic (U/L)
Transaminase-ka Glutamic-oxalacetic (U/L)
Fosfatase alkaline (mmol/L)
Kaalshiyam aayoon (mmol/L)
Fosfooraska aan dabiiciga ahayn (mg/dL)

 

3 Falanqayn iyo dood

3.1 Diludine-ka tijaabada ku jira wuxuu hagaajiyay heerka dhalmada, miisaanka ukunta, cutubka Haugh iyo miisaanka qaraabada ah ee jaallaha ukunta, taasoo muujisay in diludine-ku uu leeyahay saameynta kor u qaadista isku-darka borotiinka iyo hagaajinta xaddiga isku-darka albamka qaro weyn ee cadka ukunta iyo borotiinka jaallaha ukunta. Intaa waxaa dheer, heerka uric acid ee seeramka ayaa si cad loo dhimay; waxaana guud ahaan la qiray in hoos u dhaca ku jira nitrogen-ka aan borotiinka ahayn ee seeramka ay la macno tahay in xawaaraha catabolism-ka ee borotiinka la dhimay, waqtiga haynta nitrogen-kana dib loo dhigay. Natiijadani waxay bixisay aasaaska kordhinta haynta borotiinka, kor u qaadista dhigista ukunta iyo hagaajinta miisaanka ukunta digaagga. Natiijada baaritaanka waxay tilmaamtay in saameynta dhigiddu ay tahay mid ugu fiican marka lagu daro 150mg/kg oo diludine ah, taas oo asal ahaan la jaanqaadaysay natiijada.^[6,7]ee Bao Erqing iyo Qin Shangzhi waxaana lagu helaa iyadoo lagu daray diludine xilligii dambe ee digaagga la dhalayay. Saameyntu way yaraatay markii xaddiga diludine uu ka batay 150mg/kg, taasoo laga yaabo inay sabab u tahay isbeddelka borotiinka.^[8]waxaa saameeyay qiyaasta xad dhaafka ah iyo culayska xad dhaafka ah ee dheef-shiid kiimikaadka xubinta ee diludine-ka.

3.2 Fiirsashada Ca²⁺Serumka ukunta la dhigayo ayaa la yareeyay, P-ga ku jira seeramka ayaa la yareeyay bilowgii, dhaqdhaqaaqa ALP-na si cad ayaa loo kordhiyay marka la joogo diludine, taasoo muujinaysa in diludine uu si cad u saameeyay dheef-shiid kiimikaadka Ca iyo P. Yue Wenbin ayaa sheegtay in diludine-ku uu kor u qaadi karo nuugista^[9] walxaha macdanta Fe iyo Zn; ALP waxay inta badan ka jireen unugyada, sida beerka, lafaha, marinnada mindhicirka, kelyaha, iwm.; ALP-ga ku jira seeramka wuxuu ka yimid beerka iyo lafaha inta badan; ALP-ga ku jira lafta wuxuu ku jiray osteoblast-ka wuxuuna isku dari karaa ion-ka fosfate-ka iyo Ca2-ka ka yimaada seeramka ka dib isbeddelka isagoo dhiirrigelinaya kala-goynta fosfate-ka iyo kordhinta isku-darka ion-ka fosfate-ka, waxaana lagu shubay lafta qaab hydroxyapatite ah, iwm. si loo yareeyo Ca iyo P-ga ku jira seeramka, kaas oo la jaanqaadaya kordhinta dhumucda qolofka ukunta iyo miisaanka qaraabada ah ee qolofka ukunta ee tilmaamayaasha tayada ukunta. Intaa waxaa dheer, heerka ukunta ee jabtay iyo boqolkiiba ukunta aan caadiga ahayn ayaa si cad loo yareeyay marka la eego waxqabadka dhigista, taas oo sidoo kale sharraxday qodobkan.

3.3 Dufanka caloosha ku jira iyo dufanka beerka ee digaagga jiifa ayaa si cad loo yareeyay iyadoo lagu daray diludine cuntada, taasoo muujisay in diludine uu leeyahay saameynta xakamaynta isku-darka dufanka jirka. Intaa waxaa dheer, diludine wuxuu hagaajin karaa dhaqdhaqaaqa lipase ee seeramka marxaladda hore; dhaqdhaqaaqa lipase si cad ayaa loo kordhiyay kooxda lagu daray 100mg/kg oo diludine ah, waxaana la yareeyay waxa ku jira triglyceride iyo kolestaroolka ku jira seeramka (p<0.01), taas oo muujisay in diludine uu kor u qaadi karo kala-baxa triglyceride iyo inuu xakameeyo isku-darka kolestaroolka. Dufanka la xannibi karo sababtoo ah ensaymka dheef-shiid kiimikaadka lipid ee beerka.^[10,11], iyo yareynta kolestaroolka ku jira jaallaha ukunta ayaa sidoo kale sharraxay qodobkan [13]. Chen Jufang wuxuu sheegay in diludine-ku uu xakameyn karo sameynta dufanka xayawaanka isla markaana uu hagaajin karo boqolkiiba hilibka caatada ah ee digaagga iyo doofaarka, wuxuuna saameyn ku yeeshay daaweynta beerka dufanka leh. Natiijada baaritaanka ayaa caddaysay habkan ficilka, natiijooyinka kala-goynta iyo daawashada digaagga tijaabada ah ayaa sidoo kale caddeeyay in diludine-ku uu si cad u yareyn karo heerka beerka dufanka leh ee digaagga jiifa.

3.4 GPT iyo GOT waa laba tilmaame oo muhiim ah oo muujinaya shaqooyinka beerka iyo wadnaha, beerka iyo wadnahana way dhaawacmi karaan haddii hawlahooda ay aad u sarreeyaan. Hawlaha GPT iyo GOT ee seeramka si cad looma beddelin markii diludine lagu daray baaritaanka, taasoo muujisay in beerka iyo wadnaha aysan waxyeelloobin; intaa waxaa dheer, natiijada cabbirka ee SOD waxay muujisay in dhaqdhaqaaqa SOD ee seeramka si cad loo hagaajin karo marka diludine la isticmaalo muddo cayiman. SOD waxay tilmaamaysaa qashin-qubka ugu weyn ee xagjirka xorta ah ee superoxide ee jirka; waxay muhiim u tahay ilaalinta daacadnimada xuubka bayoolojiga, hagaajinta awoodda difaaca jirka iyo ilaalinta caafimaadka xayawaanka marka maadada SOD ee jirka ay korodho. Quh Hai, iwm. ayaa sheegay in diludine ay hagaajin karto dhaqdhaqaaqa 6-glucose phosphate dehydrogenase ee xuubka bayoolojiga iyo xasilinta unugyada [2] ee unugyada bayoolojiga. Sniedze wuxuu tilmaamay in diludine uu si cad u xakameeyay dhaqdhaqaaqa [4] ee NADPH cytochrome C reductase ka dib markii uu bartay xiriirka ka dhexeeya diludine iyo enzyme-ka khuseeya silsiladda wareejinta elektarooniga gaarka ah ee NADPH ee microsome-ka beerka jiirka. Odydents waxay sidoo kale tilmaameen in diludine uu la xiriiray [4] nidaamka oksaydhka isku dhafan iyo enzyme-ka microsomal ee la xiriira NADPH; habka ficilka diludine ka dib marka uu galo xayawaanka waa inuu door ka ciyaaro iska caabbinta oksaydhka iyo ilaalinta xuubka bayoolojiga [8] isagoo ka hortagaya dhaqdhaqaaqa wareejinta elektaroonigga enzyme-ka NADPH ee microsome-ka iyo xakamaynta habka peroxidation ee isku-dhafka lipid. Natiijada tijaabada waxay caddeysay in shaqada ilaalinta ee diludine ee xuubka bayoolojiga laga bilaabo isbeddellada dhaqdhaqaaqa SOD ilaa isbeddellada hawlaha GPT iyo GOT waxayna caddeysay natiijooyinka daraasadda ee Sniedze iyo Odydents.

 

Tixraac

1 Zhou Kai, Zhou Mingjie, Qin Zhongzhi, iwm. Daraasad ku saabsan diludine-ka lagu hagaajinayo waxqabadka taranka ee idahaJ. Cawska iyoLvestock 1994 (2): 16-17

2 Qu Hai, Lv Ye, Wang Baosheng, Saamaynta diludine-ka lagu daray cuntada maalinlaha ah si loo kordhiyo heerka uurka iyo tayada shahwada ee bakaylaha hilibka.J. Joornaalka Shiinaha ee Beeraha Bakaylaha1994(6): 6-7

3 Chen Jufang, Yin Yuejin, Liu Wanhan, iwm. Tijaabinta isticmaalka ballaaran ee diludine oo ah wax lagu daro quudintaCilmi-baarista Quudinta1993 (3): 2-4

4 Zheng Xiaozhong, Li Kelu, Yue Wenbin, iwm. Ka doodista saameynta isticmaalka iyo habka ficilka diludine sida kor u qaadida kobaca digaaggaCilmi-baarista Quudinta1995 (7): 12-13

5 Chen Jufang, Yin Yuejin, Liu Wanhan, iwm. Tijaabinta isticmaalka ballaaran ee diludine oo ah wax lagu daro quudintaCilmi-baarista Quudinta1993 (3): 2-5

6 Bao Erqing, Gao Baohua, Tijaabada diludine ee quudinta shimbiraha PekingCilmi-baarista Quudinta1992 (7): 7-8

7 Qin Shangzhi Tijaabada lagu hagaajinayo wax soo saarka digaagga hilibka nooca ah xilliga dambe ee dhalmada iyadoo la isticmaalayo diludineJoornaalka Xanaanada Xoolaha & Daawada Xoolaha ee Guangxi1993.9(2): 26-27

8 Dibner J Jl Lvey FJ ​​Borotiinka beerka iyo dheef-shiid kiimikaadka amino acid ee digaagga Sayniska Digaagga1990.69(7): 1188- 1194

9 Yue Wenbin, Zhang Jianhong, Zhao Peie, iwm. Daraasad ku saabsan ku darista diludine iyo diyaarinta Fe-Zn ee cuntada maalinlaha ah ee digaagga dhigistaQuudinta & Xoolaha1997, 18(7): 29-30

10 Mildner A na M, Steven D Clarke Bocorka fat acid synthase cloning ee DNA-da dhameystiran, qaybinta unugyada ee itsmRNA iyo xakamaynta muujinta somatotropin iyo borotiinka cuntada J Nutri 1991, 121 900

11 W alzon RL Smon C, M orishita T, iyo a I Cudurka dhiigbaxa beerka dufanka leh ee digaagga oo si xad dhaaf ah u quudiya cunto nadiif ah Hawlaha ensaymka ee la xushay iyo histology-ga beerka ee la xiriira sharafta beerka iyo waxqabadka tarankaSayniska Digaagga,1993 72(8): 1479- 1491

12 Donaldson WE Dheef-shiid kiimikaadka dufanka leh ee beerka digaagga ayaa ka jawaaba quudinta.Sayniska Digaagga. 1990, 69(7): 1183- 1187

13 Ksiazk ieu icz J. K ontecka H, ​​H ogcw sk i L Qoraal ku saabsan kolestaroolka dhiigga oo ah tilmaame cayilka jirka ee shinbirahaJoornaalka Sayniska Xayawaanka iyo Quudinta,1992, 1(3/4): 289- 294